Sample Preparation Equipment

Ultramicrotomes

Critical Point Dryer

Sputter Coater

Microwave Tissue Processor

Plasma Cleaner

 

Cryogenic Specimen Preparation

CryoSEM Preparation

Cryoultramicrotomy

Freeze Substitution

 

Acknowledging BEML facility

 

Ultramicrotomes

Ultramicrotomes can cut very thin (typically 50 to 150 nm) sections of plastic embedded biological or organic material (e.g., polymers) for examination in the transmission electron microscope. Semithin (1-5 um) sections can also be prepared for light microscopy and flat interior surfaces of materials can be exposed for examination using atomic force microscopy.

Steve operating microtome

Leica Ultracut UCT Ultramicrotome

RMC MT7 ultramicrotome

LKB Ultrotome III and V ultramicrotomes

 

Critical Point Dryer

Critical point drying eliminates the surface tension forces that distort and collapse surfaces of hydrated samples during air drying.  It is especially useful for preparing biological samples for scanning electron microscopy.

CPR Tousimis

Tousimis 931. GL

 

Sputter Coater

Sputter coating produces a conductive metal coating (usually gold-palladium) to reduce charging and increase secondary electron signal in the scanning electron microscope.

Sputter Coater Polaron

Polaron E5100

 

Microwave Tissue Processor

Accelerates fixation, dehydration, infiltration and embedding of samples for transmission electron microscopy.

Pelco Biowave Pro

Pelco Biowave Pro

 

Plasma Cleaner

Plasma cleaning is used to remove or stabilize contaminating organic material or to make surfaces more hydrophilic for better spreading of negative stain.

Plasma Cleaner Harrick Plasma

Harrick Plasma PDC-32G

 

 

Cryogenic Specimen Preparation

Includes freeze fracture/freeze etch for SEM, cryoultramicrotomy, freeze substitution, low temperature embedding, and freeze drying of thin samples.

 

CryoSEM Preparation

For freeze fracturing, freeze etching and coating SEM samples at low temperature.

Leica MED cryoSEM station

Leica EM VCT100, QSG100 and MED020

 

Cryoultramicrotomy

Low temperature ultramicrotomy of polymers or hydrated biological materials that cannot be sectioned at room temperature.

UCT with Cryo

Leica Ultracut UCT ultramicrotome with FCS cryo attachment 

 

Freeze Substitution

Freeze substitution is used to preserve molecular structure following rapid freezing of biological samples and for better preservation of tissue antigens in immune electron microscopy.

Leica EM AFS

Leica EM AFS for automated freeze substitution and low temperature embedding

Hitek metal block and liquid cryogen immersion freezing device maximize freezing rates for optimum structural preservation of samples to be prepared by freeze substitution or freeze fracture.