Leica SP8 Confocal Microscope

Overview: This is an inverted confocal microscope with five filter-free spectral and individually regulatable channels (four PMT channels and one high-sensitivity GaAsP HyD detector with photon counting capacity), and an optional fast 8 KHz resonant scanner for low photo-toxicity.

 

Specifications:

Microscope: 

• Inverted Stand DMI 6000 Bino
• Motorized stage
• Super Z Galvo stage

Lasers: 

• 405 nm Diode laser
• Argon/2 (458, 488, 514 nm)
• 561 nm DPSS laser
• 633 nm HeNe laser

Scanners: 

• Continuously adjustable non-resonant scanner (1-1800 Hz)
• Resonant scanner (8000 Hz) – 28 fps at 512 x 512

Objective:

• 10x/0.30 HC PL FLUOTAR
• 20x/0.75 HC PL APO IMM CORR CS2
• 40x/1.30 HC PL APO OIL CS2
• 63x/1.40 HC PL APO OIL CS2
• 100x/1.40 HCX PL APO OIL CS

Detectors:

• Four PMTs
• One HyD
• All filter-free spectral and individually regalatable detectors
• Transmitted light brightfield detector (with DIC option)

Software:

• LAS AF 3 for image acuqisition, processing, and quantification

 

This microscope was acquired through an NIH Instrumentation grant awarded to Prof. Akiko Nishiyama. It is part of the Advanced Light Microscopy Facility and is housed in the Bioscience Electron Microscopy Laboratory. Please contact Chris O’Connell for microscope accessing and training (new user registration, calendar access, and door access to BEML, etc).